Alkyldihydroxyacetone-P synthase. Solubilization, partial purification, new assay method, and evidence for a ping-pong mechanism.
نویسندگان
چکیده
منابع مشابه
Alkyldihydroxyacetone-P synthase. Solubilization, partial purification, new assay method, and evidence for a ping-pong mechanism.
Alkyldihydroxyacetone-P (alkyl-DHAP) synthase, the enzyme that forms the ether linkage of alkyl and alk-1-enyl glycerolipids, has been solubilized from ehrlich ascites cell microsomes using Triton X-100 and acetone precipitation. the solubilized enzyme, which is stabilized by glycerol or ethylene glycol, was then purified further by chromatography on DEAE-cellulose, QAE-Sephadex, Matrex Red, an...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34205-4